Plasmid_Backbone

Part:BBa_K5299003

Designed by: Maria Nefeli Stoupa   Group: iGEM24_Thessaly   (2024-09-22)


pSEVA23g19[g2]

pSEVA23g19[g2] is a modular plasmid from the SEVA (Standard European Vector Architecture) collection, specifically designed for genetic engineering in Gram-negative bacteria. The "23" indicates that it carries a kanamycin resistance gene for selection, while "3g" refers to the pBBR1 origin of replication, which supports stable replication across a broad range of bacterial species. The "[g2]" tag specifies that the plasmid's cargo is 1AI3, associated with level 1 Golden Standard reactions.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 333
    Illegal XbaI site found at 306
    Illegal XbaI site found at 709
    Illegal SpeI site found at 771
    Illegal PstI site found at 294
    Illegal PstI site found at 721
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 333
    Illegal SpeI site found at 771
    Illegal PstI site found at 294
    Illegal PstI site found at 721
    Illegal NotI site found at 46
    Illegal NotI site found at 739
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 333
    Illegal BamHI site found at 312
    Illegal BamHI site found at 703
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 333
    Illegal XbaI site found at 306
    Illegal XbaI site found at 709
    Illegal SpeI site found at 771
    Illegal PstI site found at 294
    Illegal PstI site found at 721
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 333
    Illegal XbaI site found at 306
    Illegal XbaI site found at 709
    Illegal SpeI site found at 771
    Illegal PstI site found at 294
    Illegal PstI site found at 721
    Illegal NgoMIV site found at 2101
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 678
    Illegal BsaI.rc site found at 94


[edit]
Categories
Parameters
None